Journal of Glycobiology
Open Access
ISSN: 2168-958X
+44 1478 350008
ISSN: 2168-958X
+44 1478 350008
Galyna A Ushakova
Posters-Accepted Abstracts: J Glycobiol
The presentation is devoted to the study of distribution of common as heparin sulfate and hyaluronate binding activity
in the rat brain for different stages of morphogenesis under normal conditions and differ type of pathogenesis
(hyperphenylalaninemia, 131I-inducable maternal hypothyroidism, low dose irradiation, somatogenic postoperative pain,
chronic pancreatitis). It was proposed the quantitative measure of common level of heparin sulfate and hyaluronate binding
activity of proteins. Due to obtained data the conception of morphogenesis events depends of level of heparin sulfate and
hyaluronate binding activity of soluble, membrane-associated, membrane and extracellular/cytoskeleton components during
brain development was proposed. The correlation of heparin sulfate-binding to hyaluronate binding activity at the early stage
of brain development should be 1:10 under normal physiological condition. It was shown the dependence of hippocampus
neuron adhesion to the level of free heparansulfate and hyaluronate in the cultures. It was indicated the heparin sulfate and
hyaluronate binding components in the nucleus of proliferating and migrating nervous cells. The membrane-associated heparin
sulfate-binding proteins from newborn rat brain (19 ??¸ 28 kDa) and membrane hyaluronate binding proteins from embryonic
human brain (250 ??¸ 20 kDa) were purified. The elevation of glycosaminoglycan binding activity was shown at the first stage of
brain morphogenesis under hyperphenylalaninemia. It was presented the proofs that heparin sulfate and hyaluronate binding
proteins take part in the neural plasticity under 131I-inducable maternal hypothyroidism, low dose irradiation (single and
fractionated in the 0.25 Gy dose), somatogenic postoperative pain, acute and chronic pancreatitis.