ISSN: 2376-0354
+44-77-2385-9429
Liu Chang
China
Research Article
Transcriptome Analysis of the Chinese Cabbage (Brassica rapa Ssp. Pekinensis) Petal Using RNA-Seq
Author(s): Liu Chang, Liu Zhiyong, Ye Xueling, Wang Yiheng, Meng Qian and Feng Hui
Liu Chang, Liu Zhiyong, Ye Xueling, Wang Yiheng, Meng Qian and Feng Hui
Flowers are the most complex and attractive organ in flowering plants that play an important role in the angiosperm reproductive process. In this study, we employed next-generation high-throughput sequencing (RNA-Seq) to gain insight into the wide range of transcriptional events that are associated with petal development in the Chinese cabbage (Brassica rapa ssp. pekinensis). We isolated RNA sequences from the vegetative mass at the four-leaf stage and petals in their full-bloom. The sequences were aligned to a reference genome and analyzed to measure gene expression levels and to detect Differentially Expressed Genes (DEGs). A total of 11,079 DEGs were obtained, of which 3,900 DEGs were up-regulated in the petal sample, and 7,179 were down-regulated. In 1,595 Specifically Expressed Genes (SEGs); 303 SEGs exhibited up-regulation with 1,292 down-regulations. Gene Ontology (GO).. View More»
DOI:
10.4172/2376-0354.1000114